RAPTOR anticorps

N° du produit ABIN492625

Cet anticorps Souris Monoclonal détecte spécifiquement RAPTOR dans WB. Il présente une réactivité envers Humain, Souris, Rat et Chien.

Aperçu rapide pour RAPTOR anticorps (ABIN492625)

Antigène: RAPTOR

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAPTOR est non-conjugé

Application: Western Blotting (WB)

Clone: 1-00E-011

Détail du produit anti-RAPTOR anticorps

Specificité: Recognizes Raptor.

Attributs du produit: Synonyms: RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycinTOR-scaffold protein

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Peptide conjugated to hemocyanin derived from Raptor Sequence.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control Provided. Cell lysate from untreated Hela cellsDescription: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Date de péremption: 12 months

Détails sur RAPTOR

Antigène: RAPTOR

Autre désignation: RAPTOR

Sujet: The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473Synonyms: KIAA1303, P150 target of rapamycin TOR-scaffold protein, RPTOR, Regulatory-associated protein of mTOR

ID gène: 57521

UniProt: Q8N122

Pathways: L'effet Warburg